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細胞処理作業における培地交換プロセスの効率化に関する研究
https://kokushikan.repo.nii.ac.jp/records/15814
https://kokushikan.repo.nii.ac.jp/records/15814259bc72e-8ac1-4de2-b529-1bc06d9e88c7
| 名前 / ファイル | ライセンス | アクション |
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本文 (6.3 MB)
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内容の要旨及び論文審査の結果の要旨 (670.3 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2023-05-06 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 細胞処理作業における培地交換プロセスの効率化に関する研究 | |||||||||||
| 言語 | ja | |||||||||||
| タイトル | ||||||||||||
| タイトル | Study on improving the efficiency of the media change in cell processing | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 著者 |
野々山, 良介
× 野々山, 良介
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| 発表年月日 | ||||||||||||
| 値 | 2023-03-20 | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Manual cell processing in the field of regenerative medicine is labor-intensive and expensive; as such, its efficiency needs improvement. Recently, automatic cell culture systems equipped with vertically articulated robots have been developed. However, automating all the cell processing tasks complicates these systems. This study aims to develop a simple and rational cell processing system by combining robot and human tasks. Herein, each cell processing task was extracted, and the division of labor between human and robot was determined. Consequently, the discarding and injecting tasks of the media change, which rely on the operator's sensory perception in particular, were selected for efficiency improvement by the robot. First, an approach to enhance the efficiency of discarding tasks was investigated. The discarding task was divided into smaller motions by utilizing the operator’s motion as a reference, and the optimal speed setting combination was determined experimentally. Evaluation experiments showed that robots could perform the discarding tasks more efficiently than humans. The operator could only evacuate the flask in the original direction, while the robot could evacuate the flask in the opposite direction, thereby reducing the risk of dripping. Second, an approach to enhance the efficiency of injecting tasks was investigated. The development of a cap equipped with an inspiration port and an injection port facilitated the injection of culture media at a constant flow rate from an injecting bottle. The weight of the culture media injected into the flask was measured in real time using a load cell. The amount to be injected during the injection end motion was predicted to calculate the injection cessation timing. Evaluation experiments showed that robots could perform the injecting tasks more efficiently than humans. Furthermore, the risk of dripping was reduced when the robot was used. Finally, we developed discarding and injecting robots that can be installed in a safety cabinet. Experiments on discarding time and injecting accuracy, employing the injecting and discarding robots, respectively, demonstrated that the results were equivalent to or better than those obtained in the case of manual operation. The discarding and injecting processes could be performed in parallel using the robots, which took less than half the time than that taken in manual operation. The robots developed herein can reduce culture costs by reducing the time required. Further, they can reduce the risk of contamination owing to dripping. The robots can be introduced into current production processes because they do not require any changes in the manual cell processing procedures. | |||||||||||
| 学位名 | ||||||||||||
| 学位名 | 博士 (工学) | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||||
| 学位授与機関識別子 | 32616 | |||||||||||
| 学位授与機関名 | 国士舘大学 | |||||||||||
| 学位授与年度 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | 令和4年度 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2023-03-20 | |||||||||||
| 報告番号 | ||||||||||||
| 値 | 乙第58号 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 乙第58号 | |||||||||||
| NDC | ||||||||||||
| 主題Scheme | NDC | |||||||||||
| 主題 | 491.11 | |||||||||||
| フォーマット | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | application/pdf | |||||||||||
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| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| キーワード | ||||||||||||
| 値 | Regenerative medicine | Media change | Cell processing system | Discarding robot | Injecting robot | |||||||||||
| 注記 | ||||||||||||
| 値 | 本研究は，テルモ株式会社より共同研究費を受けて実施致しました。 | |||||||||||
